Sentence examples for buffer and then used from inspiring English sources

The pellet fraction was solubilized with high salt buffer and then used for Spc110 enrichment.

The extracted DNA was dissolved in 100 μL TE buffer and then used as the PCR templates.

Both E. coli-derived 4 × T β4 proteins and commercial T β4 were subsequently diluted to 250 ng/ μL in 1×PBS buffer and then used to in a MTT assay.

Horseradish peroxidase-conjugated secondary donkey anti-rabbit IgG (#NA934V Amersham Biosciences, Piscataway, NJ) was diluted to 1 5,000 in blocking buffer and then used to incubate the membrane for 1 hr at room temperature.

DNA was extracted from approximately half of each egg by homogenizing each sample in PBS buffer and then using a QiaAmp DNA Mini-Kit (QIAGEN).

Cells were lysed in Passive Lysis Buffer (Promega), and then used to measure luciferase activity.

A Beckman Phi690 pH Meter (Fullerton, CA) with a Beckman Coulter combination pH electrode, Model 511275 (Fullerton, CA), was calibrated using Fisher (Fairlawn, NJ) pH 4.00 and 6.00 buffer standards and then used to measure the pH of the experimental samples.

The filters were rinsed by adding 100 μL of buffer, vortexing briefly and then using a pipet tip to "wash" the two membranes 3× each.

This effectively means that a user can overload a Direct Memory Access (DMA) buffer within the bootROM and then use it to gain high-level access before the security part of the boot process runs.

Four separate titrations were performed, including three controls, ligand into buffer, buffer into protein, and buffer into buffer, which were then used to correct the experimental titration, ligand into protein.

The concentrated cells were washed in 10 mL 67 mM phosphate buffer (pH 7.4), resuspended in the same buffer and then immediately used.