Sentence examples for buffer and then treated from inspiring English sources

The fixed cells were washed twice in phosphate buffer and then treated with 1% osmium tetroxide for 1 h.

For SEM, inner ears were fixed in 2.5% glutaraldehyde in cacodylate buffer and then treated using standard procedures.

Cell membrane was lysed by NP-40 lysis buffer and then treated with micrococcal nuclease at 37°C.

All GAS plugs were treated in lysis buffer containing 50 μg/mL lysozyme at 37°C overnight, extensively washed in washing buffer, and then treated in a solution containing 50 μg/mL protease K at 55°C overnight.

Briefly, the cells (1 × 10 cells) were suspended in 100  μl binding buffer, and then treated with 5  μl of Annexin V-PE (BD Pharmingen) and 5  μl 7-AAD before analyzed on FACSCanto II flow cytometer and BD FACSDiva software v5.0.3 (Becton Dickinson, Franklin, NJ, USA).

Complexes were captured using Protein-A magnetic beads (Pierce), washed using low- and high-stringency buffers and then treated with RNA grade Proteinase K (Invitrogen).

Sections were cut at 4 µm, deparaffinized, exposed to 30 minutes treatment in a steamer at 98°C in citrate pH 7,3 buffer for ASPM and pH 6,0 buffer for MT3 and then treated with a peroxidase blocking agent (reference S2001, DAKO, Glostrup, Denmark).

The bead pellets were washed once with ice-cold 1× wash/bind buffer while sitting on a magnetic stand, resuspended in 150 μL 1× TE buffer (pH 8.0), and then treated with Proteinase K to release methyl-CpG-enriched DNA.

For antigenic retrieval, the sections were submerged into EDTA antigenic retrieval buffer and microwaved, and then treated with 3%% hydrogen peroxide in methanol to quench endogenous peroxidase activity.

Libraries were normalized by denaturation and rehybridization in NaCl and TMAC (tetra-methyl-ammonium-chloride) buffers [ 39] and then treated with Duplex Specific Nuclease to digest cDNAs from highly abundant transcripts [ 40].

Dissected primordia were fixed for 2 h in 2 % glutaraldehyde in 0.1 M phosphate buffer (pH 7.2), rinsed in buffer for 2 h and then treated with 1%% (w : v) osmium tetroxide solution overnight at 4 °C.