Sentence examples for buffer and then transferred from inspiring English sources

Antigen retrieval was performed using high-temperature/high-pressure incubation in 0.01 mol/L citric acid buffer (pH 6.0) for 12 min. Slides were allowed to cool for 30 min in citric acid buffer and then transferred to phosphate-buffered saline (PBS) at pH 7.4.

Genomic DNA was digested with Hind III, electrophoresed on a 0.8% agarose gel in 0.5× TBE buffer, and then transferred to a nitrocellulose membrane.

Thawed specimens were pulverized in glass homogenizers, containing lysis buffer and then transferred to test tubes with protease inhibitor and centrifuged at 14000 rpm for 10 minutes.

All mixtures were loaded and separated on 5%% non-denaturing PAGE gels in 0.5 × TBE buffer and then transferred onto Hybond-N + nylon membranes (Amersham Pharmacia).

The restricted DNA fragments were separated by electrophoresis in 0.9% agarose gel in 1× TAE (0.04 M Tris-acetate, pH 7.9, 1 mM EDTA pH 8.0) buffer and then transferred onto nylon membranes (Hybond N+, GE Healthcare Life Sciences) by vacuum blotting.

After incubation, the beads were washed five times with 1 ml coupling buffer and then transferred to 0.1 M Tris HCl buffer, pH 8.0, and incubated on ice for 2 hr followed by washing with at least three cycles of buffer at alternative pHs (coupling buffer followed by 0.1 M acetic acid/sodium acetate, pH 4.0, 0.5 M NaCl).

For example, if you are writing in a word-processing program, the computer keeps the changes you are making to your file in a buffer and then transfers them to the hard disk when you use the Save command.

For histology, excised liver, kidney and pancreas were washed in phosphate buffer and then transfer to 10% formalin fixative solution for 48 h.

The resin containing the bound protein was then converted to a slurry by the addition of chitin buffer B and then transferred to two 50 mL conical tubes.

Approximately 50  μg of protein/lane were loaded and run on the polyacrylamide gel with a Tris/glycine running buffer system and then transferred onto a PVDF membrane.

When CPE was visible, the cells were fixed (1 h) in 2.5% (v/v) phosphate buffered (pH 7.2, 300 mOsmol/Kg) glutaraldehyde, rinsed in the same buffer (3×30 min) and then transferred to the CSIRO Australian Animal Health Laboratory where the cells were processed into ultrathin sections and examined by transmission electron microscopy as described previously [5].