Sentence examples for buffer and then subjected from inspiring English sources

Briefly, two-week-cultured tissue strands as well as native articular cartilage were digested in the papain buffer and then subjected to DNA quantitation assay.

The beads were washed four times with 1 ml of lysis buffer and then subjected to SDS polyacrylamide gel electrophoresis analysis.

The nuclear fraction was washed 3 times with nuclear extraction buffer and then subjected to ultrasonication.

Stimulated cells were scraped into ice-cold 10% trichloroacetic acid followed by an acetone wash, solubilized with SDS sample buffer, and then subjected to SDS-PAGE.

The complexes were then precipitated, washed, eluted with lamelli buffer and then subjected to SDS-PAGE and Western blotting analysis probing for Stat3.

The recovered complexes after the secondary immunoprecipitation were eluted with the elution buffer and then subjected to ChIP assay, as described.

Cells were lysed in 1 ml of buffer I, and then subjected to pull-down assay.

The flow-through fractions containing synovial fluid depleted of albumin and IgG were diluted 1 1 with Laemmli Sample Buffer (BioRad) and then subjected to 1D-PAGE or 2D-PAGE analysis.

After centrifugation the supernatant was discarded and the pellet was suspended in 50 mmol L−1 phosphate buffer (pH 7.0) and then subjected to sonication.

After reactions, cells were pelleted, washed with PBS buffer four times, and then subjected to fluorescent imaging.

After incubation for 48 h, cells were harvested, washed twice with ice-cold phosphate buffer saline (PBS), and then subjected to 5 µl of FITC Annexin V and 1 µl of the 100 µg/ml PI.