Sentence examples for buffer and then stored from inspiring English sources

The chips were rinsed with PBS buffer and then stored at 4°C for further use.

Proteins were further purified by gel filtration chromatography using a Superdex-200 10/300 GL column (GE Healthcare) with 20 mmol/L Tris-HCl and 50 mmol/L NaCl, pH 8.0 as running buffer and then stored at −80°C.

DNA was isolated using the standard procedure (PURAGENE) and resuspended in 300 µLs of TE buffer and then stored at −40°C until needed.

The supernatant was aspirated and discarded, and the exosome pellet was resuspended in PBS buffer and then stored at 4°C short term (1 7 days) or −20°C long term.

The supernatant was aspirated and discarded, and the exosome pellet was resuspended in PBS buffer and then stored at 4°C short term (1 7 days) or −20°C for long term.

The final precipitate was resuspended in 5 volumes of buffer-2 and then stored in liquid nitrogen.

After blood collection, brains were rapidly removed following decapitation and the hippocampus regions were excised in ice immediately and washed in ice-cold phosphate buffer saline and then stored at -80°C until use.

After that, we find the nearest triangle using the conventional z-buffer and then store a triangle id and time of this triangle to a nearest map.

The white pellet was dried at room temperature overnight for 10-15 min. The DNA pellet was suspended in 50 μl of TE buffer (pH = 8) and then stored at -20°C.

The purified enzyme in the elution buffer (1 M imidazole, 0.5 M NaCl, 20 mM Tris HCl, pH 7.9) was further dialyzed three times in phosphate buffer (100 mM, pH 6.0), and then stored at 4 °C for further experiments.

Finally, the plugs were washed with 5 ml TE-buffer at 37°C (2 × 30 min), and then stored in TE-buffer at 4°C until further use.