Sentence examples for buffer and then stained from inspiring English sources

Cells were either left intact or permeabilized with freshly prepared 0.5% saponin (EMD Millipore) in FCM buffer, and then stained for PEG-HCCs with an anti-PEG antibody and analyzed by FCM (Supplementary Figs S1 and S2).

Gel (acrylamide mass fraction 15%) was run in 0.01 KMgTB buffer and then stained with Sybr Green I dye.

A replica gel containing xylan (0.5%) was incubated at 50°C for 5 min in buffer and then stained in 0.1% Congo Red solution.

The spores were subsequently fixed with 0.2% oxymethylene, washed twice with PBS buffer, and then stained with 30 μg/mL propidium iodide (PI; Beyotime, China) at room temperature for 30 min.

After being centrifuged at 10,000 rpm, the pellets of palladium-loaded bacteria were resuspended in 15 mL of 0.1 M Na-cacodylate buffer and then stained for an hour in the 1% osmium tetroxide solution in 0.1 M phosphate buffer at pH 7 for the measurement of the TEM.

Cells were washed with Stain Buffer and then stained with APC goat-anti-mouse IgG secondary antibody (BD Biosciences) for 20 minutes.

The pellet was re-suspended in incubation-buffer and then stained with FITC-conjugated monoclonal active caspase-3 antibody (Cell Signaling, USA).

The gels were then incubated overnight at 37°C with 0.1% glycine buffer pH 7.5 and then stained with 0.5% Coomassie Brilliant Blue R250 and destained.

For plasmid and IBB / plasmid binding assay analysis 0.8%% agarose gel in TBE buffer was used, and then stained with ethidium bromide.

On each day (0 to 10 inclusively), 5 mice were sacrificed, splenocytes were harvested, RBCs were lysed using 1 × RBC Lysis buffer (eBioscience), counted and then stained with anti-NK1.1, anti-Mac1 and anti-Gr1, or with anti-CD3ε and anti-CD4 then fixed and stained with anti-FoxP3 as per manufacturer's recommendation (eBioscience).

For transmission electron microscopy (TEM) pellets of purified granules were fixed in Karnovsky fixative (2.5% glutaraldehyde, 2% paraformaldehyde, in 0.1 M, cacodylate buffer, pH 7.2) for 2 h, post-fixed in 1% osmium tetroxide in the same buffer for 1 h and then stained en bloc with 0.5% aqueous uranyl acetate, dehydrated in acetone, and embedded in Spurr's low viscosity embedding medium.