Sentence examples for buffer and then sonicated from inspiring English sources

The pellet was resuspended in 5 ml TEN buffer and then sonicated.

Cell suspensions were then mixed with 167 µl 4X of sample buffer and then sonicated before electrophoresis of samples as described above.

and resuspended in 0.5 ml Laemmli buffer and then sonicated for 5 min for SDS-PAGE.

The pellets were washed twice with homogenization buffer and then sonicated in homogenization buffer to obtain the mitochondrial fractions.

To isolate protein extracts from primary hepatocytes, cells were washed twice with iced cold PBS, mixed with RIPA Lysis buffer, and then sonicated on ice for 20 s prior to collecting protein solution.

Serum samples of blood from the trapped animals were withdrawn by cardiac puncture and evaluated by enzyme-linked immunosorbent assay (ELISA) on plaques directly coated with antigen from cells infected with PUUV or controls, lysed in triton-borate buffer, and then sonicated.

The cells were harvested using 1.5 ml Eppendorf tube and resuspended using buffer A, and then sonicated in an ice bath.

The pellet was suspended in 10 mL of fresh FA lysis buffer + PI and then sonicated 6 times at 65% to an average size of 500 bp.

Cells were spun down at 7,459 × g for 20 min, resuspended in 1 × phosphate-buffered saline buffer (PBS, pH 7.4), and then sonicated to liberate the cell contents.

The cells harboring pET28a-6 × his-4 × T β4 were collected by centrifugation after IPTG (1 mM) induction at 37°C for 6 h, and the cells pellet was resuspended in PBS buffer (v : v = 1 : 5) and then sonicated at 200 300 W in an ice bath 6 times (pause/sonication = 10 s/10 s per time).

Synchronized L4 worms were washed in water, pelleted, frozen, and then sonicated in buffer containing EDTA as previously described (Benedetto et al. 2010).