Sentence examples for buffer and then reacted from inspiring English sources

Briefly, blots were blocked with appropriate blocking buffer and then reacted at 4°C with primary antibodies at dilutions as per the manufacturer's directions overnight.

Cells were washed in PBS, resuspended in Aldefluor buffer, and then reacted with Aldefluor substrate (STEMCELL Technologies, Vancouver, BC, Canada) according to the manufacturer's instructions.

The membranes were incubated for 1 hour with 1× blocking buffer and then reacted overnight with anti-talin antibody diluted 1 500 in blocking solution.

15-micrometer cryosections of VL and SOL muscle were incubated with a 1 : 400 dilution of mouse monoclonal antibody against fast myosin heavy chain (Clone MY-32, Sigma-Aldrich) in 0.3% BSA in phosphate buffered saline (PBS) and then reacted with a 1 : 2000 dilution of horseradish peroxidase-conjugated anti-mouse IgG (ICN).

(3) Sections reacted with a Biotin-labeled cRNA probe were incubated for 30 min with POD-conjugated streptavidin (Perkin Elmer, 1 500 in antibody incubation buffer) rinsed in washing buffer (3 × 5 min) and then reacted with TSA Cy3 working solution for 7 min.

Viruses (protein of 300 ng) were incubated in each buffer at 37°C for 10 min and then reacted by addition of 5.0 µl of 2 mM 4MU-Neu5Ac at 37°C for 30 min. The reaction was stopped by addition of 100 µl of 100 mM sodium carbonate buffer (pH 10.7).

Membranes were washed with Tris-buffer saline buffer containing 0.1% Tween 20, incubated for one hour at room temperature with primary antibodies, washed, and then reacted with peroxidase-conjugated secondary antibodies.

The membrane was washed twice in 20 mM Tris-HCl (pH 7.5), 500 mM NaCl and 0.05% Tween-20, and then reacted with 4-chloro-1-naphthol Bio-Rad Laboratoriess) in HRP color development buffer Bio-Rad Laboratoriess) for 30 min at room temperature.

The membrane was washed and then reacted with secondary HRP-conjugated antibodies (see below).

The resulting acids were transformed to diazoketones and then reacted with hydrogen bromide.

For us, we have to wait and then react.