Sentence examples for buffer and then postfixed from inspiring English sources

After fixation the embryos were washed three times in 0.2 M cacodylate buffer and then postfixed in 1% osmium tetroxide for one hour at room temperature.

Small portions of brain and liver (<1 mm3) were processed for transmission electron microscopy (TEM) immediately after euthanasia; fixed in 2.5% glutaraldehyde in 0.1M sodium (pH 7.0) cacodylate buffer and then postfixed with 2% osmium tetraoxide (OsO4), dehydrated through graded ethanols and embedded in Spurr's Resin.

After fixation, the specimens were thoroughly washed in 0.1 M cacodylate buffer and then postfixed with 1% osmium tetroxide in the same buffer for 1 h at room temperature, stained en bloc with 2% uranyl acetate in distilled water for 15 min, dehydrated in graded acetonitrile, and embedded in Epon.

Samples were rinsed in the same buffer and then postfixed with 1% osmium tetroxide and 1% potassium ferrocyanide in 0.1 M cacodylate buffer for 1 h at room temperature to enhance the staining of cytoplasmic membranes.

The cells were rinsed with three changes of cold 0.1 M sodium cacodylate buffer (pH = 6.8) by centrifugation and resuspended in fresh buffer and then postfixed with 2% OsO4 in the same buffer for 2 hr on ice in the dark.

After fixation, the specimens were thoroughly washed in 0.1 M cacodylate buffer and then postfixed with 1% osmium tetroxide in the same buffer for 1 h at room temperature, stained with 2% uranyl acetate in distilled water for 15 min, dehydrated in graded acetonitrile, and embedded in Epon.

Cells were plated on matrigel and fixed in 4% buffered glutaraldehyde and then postfixed in 1% osmium tetroxide.

Samples were washed twice with sodium cacodylate buffer (pH 7.4) and then postfixed with 1% osmium tetroxide for 1 h at 4°C.

For SEM, embryos were fixed in 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.2) and then postfixed in osmium tetroxide.

Samples for SEM analysis were fixed in 2.5% glutaraldehyde (Sigma-Aldrich) in PBS solution (0.1 M, pH = 7.4) for 3 hours at 4°C, washed three times in the same buffer (10 min each), and then postfixed with osmium tetroxide solution (1% in 0.1 M phosphate buffer, pH = 7.2).

For TEM, metamorphic stages, newly formed juveniles, and 4-day-old juveniles of P. harmeri were fixed at 4 °C in 2.5 % glutaraldehyde in 0.05 M cacodylate buffer containing 21 mg/ml NaCl and then postfixed in 2 % osmium tetroxide in the same buffer containing 23 mg/ml NaCl.