Sentence examples for buffer and then loaded from inspiring English sources

Extracted proteins were denatured by boiling for 4 min in reducing sample buffer and then loaded on 10% sodium dodecyl sulfate-polyacrylamide electrophoresis gels.

Proteins were heated at 75°C for 5 minutes in SDS-samples buffer and then loaded along with the Novex Sharp Pre-Stained Protein Molecular Weight Standard (Invitrogen) onto a 4 12 Bis-Tris NuPAGE gel (Invitrogen) and run at 125 volts.

DNA (6 pmol) was mixed with 6 μL of 6× gel loading buffer and then loaded into the wells.

Protein samples were combined with Laemmli sample buffer and then loaded on 10% Criterion™ Tris HCl Precast Gels (from Bio-Rad) and run at a constant voltage.

The spin column flow-through was combined with nonreducing SDS PAGE sample loading buffer and then loaded on a nonreducing 14% SDS PAGE gel.

One ml of the sample was diluted 20 times in 1.7 M Ammonium Sulfate buffer and then loaded on to the Hydrophobic interaction column column (1 ml Phenyl Sepharose Fast Flow, low substitution, from GE) at 1 ml/min.

Samples of FAM-labeled dsDNAs and DBD were incubated for 10 min at room temperature in buffer HN50 and then loaded on 8% acrylamide/bisacrylamide 1× TBE mini gels with running buffer prechilled at 4 °C.

The resulting concentrate was dissolved in buffer A, and then loaded on a Sephacryl S-100 gel filtration column (1.6 cm × 60 cm).

The collected fractions were dialyzed against 20 mM phosphate buffer, pH 6.5 and then loaded onto a Cellufine Sulphate column, which had been equilibrated with the same buffer.

The resulting peak was collected and dialyzed versus 10 mM HEPES buffer pH 8 and then loaded on a MonoQ™ column (Pharmacia) and eluted with a 20 mM to 500 mM NaCl gradient.

Samples were mixed with an equal volume of 2 × Laemmli buffer, boiled (5 min), and then loaded onto a 12% polyacrylamide gel.