Sentence examples for buffer and then fractionated from inspiring English sources

Tissue lysates were boiled with 6 × sodium dodecyl sulfate (SDS) loading buffer and then fractionated by SDS-PAGE.

After 4 washes with IPP, the beads were spun down, boiled for 5 min in 30 μl of sample buffer, and then fractionated by 10% SDS-polyacrylamide gel electrophoresis.

The precipitated gfp uv was collected and re-suspended in extraction buffer (TE, with added EDTA) and then fractionated on to the HIC column.

Cell extracts and conditioned medium were diluted fourfold in lysis buffer and reduced with 5% β-mercaptoethanol and then fractionated by PAGE and analysed by Western blotting as previously described (Chase et al., 2002).

Polymerase chain reaction products were digested with the restriction enzyme Nco I and then fractionated by gel electrophoresis.

Briefly, proteins were solubilized and then fractionated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).

This mixture was heated to 95 °C for 4.5 h and then fractionated with 1000 and 5000 Da membranes.

The plasma is tested for H.I.V., hepatitis and other pathogens, and is then "fractionated" using a combination of ethanol and salt concentrations to yield a few drops of antibodies, which are proteins.

T. indica seeds were extracted with methanol and were then fractionated with different compositions of hexane, ethyl acetate and methanol.

In brief, cells were lysed in EBC buffer (50 mM Tris [pH 8.0], 120 mM NaCl, 0.5% NP-40) supplemented with a cocktail of protease and phosphatase inhibitors (Roche), boiled in sodium dodecyl sulphate (SDS -containing SDS -containingand then sampleractionated buffer–Pand.

Again, these fusions were expressed in S. coelicolor using thiostrepton; protoplasts prepared and lysed then fractionated using ultracentrifugation.