Sentence examples for buffer and then fixed from inspiring English sources

The next day, the cells were washed in 1× HEPES-buffered Hank's balanced salt solution (HEPES/HANKS) buffer and then fixed in 1% paraformaldehyde for 30 min.

Subsequently, the pellets were rinsed in buffer and then fixed in 2 % OsO4 in the same buffer for 1.5 h.

Cells were washed with phosphate buffer and then fixed with 2% paraformaldehyde.

Cells were washed twice with BD FACS wash buffer and then fixed with 1% methanol free formaldehyde.

Then the cells were washed for three times with FACS buffer and then fixed with 1% Paraformaldehyde.

Embryos (E10.5 E12.5) were washed in unadulterated buffer and then fixed for 30 45 min in buffer containing 0.2% glutaraldehyde, 5 mM EGTA, and 2 mM MgCl2.

After intracytoplasmic cytokine staining, the cells were washed twice with 200μL of FACS perm-buffer and FACS buffer, respectively, and then fixed in 200μL of FACS FIX Solution (10 g/L of paraformaldehyde, 10.2 g/L of sodium cacodilate and 6.63 g/L of sodium chloride, pH 7.2, all from Sigma, St Louis, MO, USA).

After the treatment, the cells were washed twice for 2 min in the buffer alone and then fixed in the buffer containing 1% formalin for 10 min. The treating solution was removed and the cells were washed twice with HBSS solution.

For immunofluorescence, coverslips were washed once in cytoskeleton buffer (65) and then fixed for 10 min in 4% EM-grade formaldehyde (Polysciences) in cytoskeleton buffer.

The hearts were perfused for another 5 minutes with buffer alone and then fixed under pressure for 10 minutes with 4% paraformaldehyde (pH 7.4) (PFA).

Cells were washed twice in cacodylate buffer pH 7.0 and then fixed in a solution containing 2.5% glutaraldehyde, 4% paraformaldehyde, and CaCl2 (25 mg/mL), placed on the poly-L-lysine-coated coverslip and dehydrated in growing concentrations of ethanol.