Sentence examples for buffer and then eluted from inspiring English sources

After incubation at 4°C overnight with gentle shaking, the resin was extensively washed with the wash buffer and then eluted with the elution buffer (20 mM Tris-HCl, pH 7.8, 400 mM NaCl, 300 mM imidazole and 0.2% DPC).

The column was washed with 5 bed-volume of amylose resin wash buffer and then eluted with 10 bed-volume of amylose resin elution buffer (20 mM maltose in amylose resin wash buffer) followed by 0.5 M NaOH wash of the column.

The precipitated beads were washed at least four times with the protein extraction buffer and then eluted by 2 × SDS protein-loading buffer with boiling for 5 min.

The sample was rinsed with the same buffer and then eluted with the same buffer containing 0.01 M NaCl at a flow rate of 20 ml/h.

The column was rinsed thoroughly with the same buffer and then eluted with 100 mM methyl-α-d-mannoside in the buffer at a flow rate of 20 ml/h.

The column was washed with 4 ml of the same buffer and then eluted using a 20 mL gradient of NaCl (0 0.5 M) at a flow rate of 0.5 ml/min.

DNA (35 μg) was bound to the CXXC matrix in a 100 mM NaCl-containing column buffer, washed and then eluted using buffer containing 1 M NaCl.

The F3 sample, which included Cel7A and Bgl3A, was applied to a Source 15S cation-exchange column equilibrated with buffer B and then eluted with the same buffer.

Digests were loaded in 2% acetonitrile/0.1% formic acid (Buffer A) and then eluted with a linear gradient of buffer B (100%acetonitrile/0.1%% formic acid) at 300 nL/min.

The columns were washed with 8 ml of 10 and 20 mM imidazole in buffer A and then eluted step-wise with 2.5 ml aliquots of buffer A containing 50, 100, 250, and 500 mM imidazole.

After the cation exchange column was removed away, the protein was washed with 40 ml buffer A, and then eluted using a 0 1.0 M NaCl gradient in the same buffer with a speed of 1 ml/min.