Sentence examples for buffer and then diluted from inspiring English sources

The extracted DNA was suspended in 50 µl TE buffer, and then diluted 1∶20 for use in PCR reactions.

Samples were eluted in 75 μl EB buffer and then diluted 1 5 in TE buffer.

The cell lysates were precipitated with 100 μl streptavidin T1 magnetic beads, eluted with 50 μl nonreducing SDS-PAGE loading buffer and then diluted with cold RIPA buffer to a final volume of 1 ml and was precipitated with 100 μl (1 × 10) 2D3-conjugated M-270 dynabeads, then eluted with 100 μl nonreducing loading buffer.

Filtered protein stock solutions were each prepared in the same buffer solution and then diluted for injection at the desired concentrations.

The formic acid extracts were neutralized with 1 M Tris phosphate buffer (pH 11) and then diluted with the ELISA sample buffer (1 : 20).

For analyzing adiponectin levels in fat tissue, extracted proteins were first adjusted to a concentration of 0.5 μg μL−1 in a total volume of 60 μL in the same extraction buffer (T-PER) and then diluted to a concentration of 0.0025 μg μL−1 using the diluent buffer (1X) from the kit.

Twenty embryos were homogenized in 150 μl of 0.5% trichloroacetic acid, neutralized by 1 M Tris-acetate buffer, pH 7.75 and then diluted to 1.5 ml.

The erythrocytes were washed twice with phosphate buffered saline (PBS) and then diluted to 2% (v/v) erythrocytes solutions with PBS.

Primers were dissolved in TE buffer at 100 µM and then diluted to a working concentration of 20 µM with water.

Plasmids were purified with Endo free Maxi-Prep purification kits (EndoFree Plasmid Maxi Kit, QIAgen) using pyrogen-free material and eluted in pyrogen-free TE buffer in 200 µl/column and then diluted for injection in sterile PBS.

Extracts were sonicated to generate 200 600 bp DNA fragments, incubated on ice for 20 min, centrifuged at 16,000 × g for 10 min, and then diluted 1 10 with dilution buffer (0.01% SDS, 1.1% Triton X-100, 1.2 mM EDTA, 16.7 mM Tris pH 8 and 167 mM NaCl).