Sentence examples for buffer and then denatured from inspiring English sources
The immunoprecipitates were washed three times in lysis buffer and then denatured in Lemmli's buffer prior to SDS-PAGE.
For mRNA-sequencing, the library was diluted to 10 nM in EB buffer and then denatured using the Illumina protocol.
The immunoprecipitates were washed thrice in lysis buffer and then denatured in Laemmli buffer before SDS PAGE.
The proteins extracted from peanut samples were mixed with SDS loading buffer, and then denatured at 100 °C for 5 min.
To detect polyubiquitinated material, cells cultured in 12-well dishes were lysed in 50 μl of IP buffer and then denatured in SDS/PAGE sample buffer at 70°C for 10 min.
After extraction with phenol-chloroform and precipitation with ethanol, DNA fragments were resuspended in 80% formamide in 1x TE buffer and then denatured for 5 min at 95°C before separation by electrophoresis using 8% urea-polyacrylamide sequencing gels.
After 2 h, cells were collected directly in 100 μL protein sample buffer (#161-0737EDU, BioRand and then denatured at 90 °C for 10 min. Individual samples of 15 μL were loaded in each lane.
FFPE 4 µm kidney sections were deparaffinized in xylene, dehydrated in ethanol, pretreated in citrate buffer (pH 6.8), digested with pepsin, and then denatured.
Most of the functional ScFv was generated when the inclusion bodies were solubilized in a detergent, air oxidised in the presence of CuSO4 and then denatured in urea buffer in comparison to other protocols.
Dig-labeled A10-2 were synthesized in Invitrogen Biotech Co., Ltd., dissolved in binding buffer at 40 ng/ μL concentration and then denatured for 5 min at 95°C, cooled for 5 min at 4°C, and then placed at RT for 5 min.
After UV treatment and PR, cellular DNA was isolated and then denatured in TE buffer (10 mM Tris-CL and 1 mM EDTA, pH 7.5) by boiling for 5 min. Thesamples were dot-blotted onto a Hybond N+ membrane (Amersham, NJ) using 200 ng of DNA for the CPD assay and 1 μg of DNA for the 6-4PP assay.
