Sentence examples for buffer and then boiling from inspiring English sources

The binding reaction was stopped by adding 2 μL of 6× Laemmli's SDS sample buffer and then boiling at 100°C for 5 min.

For western blotting assay, the protein samples were denatured by mixing with equal volume of 2 × sample loading buffer and then boiling at 100 °C for 5 min.

After being treated with different DNA sequences, cells were washed several times with PBS, and harvested by SDS-loading sample buffer, and then boiled.

Protein A-agarose beads were washed in lysis buffer and then boiled in SDS sample buffer with 100 mM DTT.

The beads were washed three times with 20 volumes of lysis buffer and then boiled in denaturing sample loading buffer.

After incubation at 4°C for 10 min, the beads were washed five times in RHPA buffer and then boiled in SDS loading buffer.

Beads were washed 2× with kinase buffer, and then boiled in 2× SDS-PAGE loading buffer, prior to loading on the gel and subsequent Western blotting.

Beads were washed in lysis buffer and then boiled for 10 min in SDS Loading Buffer.

Immunoprecipitates were washed 3 times with lysis buffer, and then boiled in SDS buffer for 5 min at 100°C.

The resin was separated by centrifugation, washed 5 times with ice-cold lysis buffer, and then boiled in SDS sample buffer.

Complexes were centrifuged at 14, 000 × g and washed 5 times in wash buffer before addition of 20uL 2× Laemmli buffer and then boiled for 5 minutes.