Sentence examples for buffer and then boiled from inspiring English sources

After being treated with different DNA sequences, cells were washed several times with PBS, and harvested by SDS-loading sample buffer, and then boiled.

Protein A-agarose beads were washed in lysis buffer and then boiled in SDS sample buffer with 100 mM DTT.

The beads were washed three times with 20 volumes of lysis buffer and then boiled in denaturing sample loading buffer.

Beads were washed 2× with kinase buffer, and then boiled in 2× SDS-PAGE loading buffer, prior to loading on the gel and subsequent Western blotting.

After incubation at 4°C for 10 min, the beads were washed five times in RHPA buffer and then boiled in SDS loading buffer.

Beads were washed in lysis buffer and then boiled for 10 min in SDS Loading Buffer.

For western blotting assay, the protein samples were denatured by mixing with equal volume of 2 × sample loading buffer and then boiling at 100 °C for 5 min.

The binding reaction was stopped by adding 2 μL of 6× Laemmli's SDS sample buffer and then boiling at 100°C for 5 min.

siRNA were synthetized and annealed by Eurogentec (Belgium), sequences targeting A. carolinensis are presented in Fig.  4a and the Si-control (si-luciferase) sequence was: CGTACGCGGAATACTTCGA. Brains (stored at −80 °C) were dissected, lysed in laemmli sample buffer, sonicated and then boiled at 95 °C for 8 min.

Wash buffer was removed and sample buffer was added, and then boiled for 5 min at 95°C for immunoblotting.

The beads were exhaustively washed in the ice-cold buffer described above, and then boiled in SDS-loading buffer.