Sentence examples for buffer and then bathed from inspiring English sources

Cells were washed with modified Krebs Ringer buffer and then bathed in a Ca2+-free medium, containing EGTA (100 µM).

Prior to imaging, cells were washed with 1.5 mL of Dulbecco's Phosphate Buffered Saline (DPBS) and then bathed in 3.0 mL of DMEM without phenol red indicator supplemented with 1% penicillin/streptomycin during imaging.

Since then he has undergone more surgery and chemotherapy or, in his words, been "filleted, disemboweled and then bathed in hot poison".

SAN DIEGO — This is cancer therapy at its most aggressive, a treatment patients liken to being filleted, disemboweled and then bathed in hot poison.

Twenty milliliters of nGO-PEG solution (approximately 0.5 mg/mL) was transferred to a sealed glass bottle and then bathed at different temperature for 24 h or bathed at 90°C for different times.

After that, 50 mL of the NGO-PEG solution (1 mg mL−1) was transferred to a sealed glass bottle and then bathed at 90 °C for 24 h.

Cerebral or buccal ganglia were dissected, and then bathed in protease (Sigma, Israel) for 2 hours.

The membranes were washed as above and then bathed in ECL western blotting detection reagent (Amersham, GE Healthcare) at 0.125 ml/cm2 for 1 minute.

Two-day-old cerebellar explants were loaded for 30 min at 37°C with 1 2 μM Flou-3-AM (Molecular Probes, Invitrogen) and then bathed in DPBS.

We then prepared live larval fillets in physiological solution and then bathed the NMJ in the actin depolymerizing drug Latrunculin A (LAT A) and compared the results with vehicle-only controls (see Materials and Methods).

Let the solution sit on the coat for 10 minutes and then bathe her in her regular shampoo.