Sentence examples for buffer and then analyzed from inspiring English sources

Immunoprecipitates were washed 3 times with lysis buffer and then analyzed by immunoblotting.

All suspensions were washed three times with PBS, suspended in 500 μl of binding buffer, and then analyzed by flow cytometry (Epics XL, Beckman Coulter, USA) using FLOWJO 7.6 software.

Cells were subsequently washed, resuspended in FACS buffer, and then analyzed using a six-colour BD FACSCanto flow cytometer (BD Biosciences).

Proteins were resuspended in SDS-polyacrylamide gel electrophoresis (PAGE) sample buffer, and then analyzed by SDS-PAGE as described previously [58].

The cells were suspended in binding buffer and then analyzed by flow cytometry.

Cells were resuspended in 250 μl of staining buffer and then analyzed.

The formulations were suspended in phosphate buffer (pH 7.4) and then analyzed.

The protein was deuterated in different pH* buffers (4.5 9.0), digested, and then analyzed by LC MS/MS.

To examine the rTFF1 polymeric forms, the proteins were treated with phosphate buffer (pH 7.0) and Na2HPO4-citric acid buffer (pH 2.4) [ 38], and then analyzed by 14% native PAGE [ 39].

Cells were washed and stained with FITC- anti-mouse IFN-γ, PE-anti-mouse IFN-γ, PE anti-mouse IL-10 or isotype controls (BD Biosciences Pharmingen) in FACS buffer with 0.5% saponin for 45 min at R.T. Cells were then washed in FACS buffer with 0.1% saponin and subsequently with FACS buffer with 0.01% saponin and then analyzed on a FACScan flow cytometer with the Cell Quest software program.

Pellets were washed four times in lysis buffer, boiled for 10 min, and then analyzed by Western blot (Latz et al, 2004).