Sentence examples for buffer and then analysed from inspiring English sources

After 30 min incubation at 4°C, the cells were washed twice with FACS buffer, and were then incubated with FITC labelled anti-rat IgG (BD Pharmingen) at 10 µg/ml in FACS buffer for 30 minutes at 4°C, washed twice in FACS buffer and then analysed on a FACScalibur™ analyser (Becton Dickinson) running Cellquest™ software.

Cells were washed once with permeabilisation buffer and once with FACS buffer, and then analysed by flow cytometry.

To test the stability of the functionalizing coating, the scaffolds were incubated for 24 h at 37°C in physiologic buffer and then analysed by fluorescence microscopy and FTIR.

Kidneys and cell lines were lysed in RIPA buffer, total extracts were quantified using the Bradford reagent (BioRad) and then analysed by SDS PAGE.

The harvested cells were incubated in binding buffer with Annexin V and propidium iodide for 15 min and then analysed using an Accuri C6 flow cytometer (Accuri Cytometers Inc., Ann Arbor, MI, USA).

Annexin V-FITC and PI (5  μl per 100  μl buffer) were added for 15 min at room temperature (RT) in darkness, and then analysed by flow cytometry (Becton Dickinson, Franklin Lakes, NJ, USA) within 1 h.

Elastin in Tris buffer was deposited separately on 10 glass substrates, incubated for 72 hours at 37°C, and then analysed by scanning electronic microscope [ 21] to evaluate the protein fibres assembly.

These were digitised and then analysed.

The beads were washed again three times with a cold lysis buffer A. The proteins bound to the beads were eluted by boiling in a SDS sample buffer and then subjected to immunoblot analyses as indicated in Fig. 1c.

One hundred millilitres of aliquots, along with tenfold serial dilutions of the resulting phosphate buffer solution were then analysed using the membrane filtration technique according to standard methods (US EPA 2002a, b).

Sepharose beads were washed 3 times with 1 ml NT2 buffer and then resuspended in sample buffer for SDS-PAGE.