Sentence examples for buffer and then added from inspiring English sources

Purified P22sTsp was buffer-exchanged by size exclusion chromatography (Superdex™ 200 column, GE Healthcare) using PBS as the equilibration buffer and then added to the molten motility media just before pouring them into plates (50°C).

Trypsin (Promega) was activated by 15 min incubation at 30°C in activation buffer and then added 1∶200 to the samples, digestion was allowed to proceed over night at 37°C.

Beads were washed 3 times with NP-40 buffer and then added to cell lysates.

For affinity purification, anti-DDK agarose beads were first balanced with RIPA buffer, and then added to pre-cleared overexpression lysates for overnight incubation.

The blood samples were mixed with an equal volume of Hank's buffer and then added into centrifuge tubes that contained appropriate amounts of lymphocyte separation medium.

Therefore, the enzyme samples for the Cel7B ELISA were not heated but directly diluted in PBS buffer and then added to the wells.

Spheres were removed from the oil bath and vigorously rinsed with TRIS buffer via several buffer exchanges, and then added to a final solution of TRIS buffer.

The ISR clears an 8-bit audio sample buffer and then adds the current sample from the tables corresponding to any present ball bearings (stored as 1s in the bearing table 2D array).

But later "by first allowing CtpS to polymerize in activity buffer and then adding 1 mM UTP and ATP".

A plot of receptor concentration versus absorbance at 445 nm yields a binding curve, which was fitted in Origin using a 1 1 binding model described by Hargrove et al.[ 34] Anion-binding constants were determined by preparing solutions containing a 1 1 ratio of 3 and PV (at 50 μ m) in HEPES buffer, and then adding increasing amounts of either arsenate or phosphate working solution.

1 2 mg of every compound to be determined their ability to pass the brain barrier were dissolved in 250 µL of DMSO and 4750 µL of PBS pH = 7.4 buffer, filtered and then added to the donor 96-well plate.