Sentence examples for buffer and the total from inspiring English sources

Cells were lysed in 1× SDS-PAGE sample buffer, and the total protein was quantified using the BCA protein assay reagent (Thermo Fisher, Grand Island, NY, USA).

We selected the trees with dbh > 10 cm and computed AGB per hectare for the largest tree in the buffer and the total AGB per hectare for all trees in the buffer.

Cell lysates were prepared with RIPA buffer and the total proteins were quantified by BCA assay as mentioned above.

aRNA was fragmented using 5x fragmentation buffer and the total amount of fragmented biotinylated aRNA used per chip was 6.5 μg.

The beads were incubated at 94°C for 5 min in 1 × Laemmli's gel sample buffer and the total was loaded on a 10% SDS-polyacrylamide gel.

The concentration of GdnHCl was diluted to 0.4 M using RIPA buffer, and the total volume of each sample was made equal by adding 0.4 M GdnHCl.

The same buffer system was used for both buffers and the total buffer volume was kept constant.

Columns were washed in the manufacturer's supplied buffers and the total DNA was eluted in 200 μl elution buffer.

In particular, we studied how the response time is affected by the quantum interval, the buffer size, and the total number of terminals when different queuing disciplines are employed in the operation of the round-robin queue with a limited buffer.

Then the solution was replaced with fresh buffer solutions and the total volume maintains the same.

The total population and dwelling counts were calculated as the ratio of each DA that fell within a specified buffer area and the total area of that DA multiplied by total population/dwelling counts of their respective DA.