Sentence examples for buffer and the tissue from inspiring English sources

Samples were suspended in 0.05% RapiGest (Waters, Milford, MA, USA) buffer, and the tissue was homogenized and sonicated for protein extraction.

Generally, 39 breast cancer tissues were homogenized in 800 μl detergent lysis buffer and then the tissue homogenates were centrifuged at 12,000 g for 15 minutes to get the supernatant.

The breast cancer specimens used in this study were fixed with 4% buffered formalin, and the tissue was then embedded in paraffin.

Slides were removed from labelling buffer and excess buffer surrounding the tissue was wiped away.

For the histopathological analysis of these organs, the samples were fixed in 10% buffered formalin, and the tissues were processed by conventional techniques in 5-mm-thick paraffin slices.

RNAlater was replaced in the 2 ml plastic tube with 1.0 ml of RLT buffer (Qiagen GmbH., Germany), and the tissue was homogenized by adding a 5 mm diameter Zirconium bead (Funakoshi, Japan) and shaking with a MixerMill 300 (Qiagen GmbH., Germany) at a speed of 20 Hz for 5 min (only the outermost row of the shaker box was used).

By implementing measures to maximize the amount of buffer infiltrated into the tissue and performing centrifugation until the tissue is restored to its fresh weight, we show for the first time that three rounds of VI-C are sufficient for recovering almost all of the activity of secreted proteins into AWF extracts characterized by remarkable purity and concentration improvements.

The assay buffer was decanted and the tissues were bleached in 95% EtOH and photographed.

The lumen was first flushed with EDTA PBS buffer, and then, tissue was collected and homogenized in PBS buffer and analyzed as unit per milligram protein (a).

c-KIT: Antigen retrieval was done by immersion of tissues in Tris/EDTA buffer at pH 9.1 and heating the tissue and buffer as described for PLAP.

In brief, the staining protocol was as follows (a detailed protocol may be obtained from the corresponding author): PLAP: Antigen retrieval was done by immersion of tissues in a 0.5 M EDTA buffer at pH 8.0 and heating the tissue and buffer to 92 °C for 5 minutes in a microwave oven at 750 watts.