In the study groups (PMN-NaCl, PMN-Ri-Ac, PMN-6% HES, PMN-10% HES and PMN-Gel, n = 6 each, protocol 2B), the perfusion mode switched to a 1 1 mixture of buffer and the respective test solution after the first equilibration interval.
In the study groups (TF-NaCl, TF-Ri-Ac, TF-6% HES, TF-10% HES and TF-Gel, n = 6 each, protocol 1B), the perfusion was switched after 15 minutes to a 1 1 mixture of buffer and the respective test solution following the basal measurement of transudate formation.
After that, basal perfusion was continued (HP-Control, n = 6, protocol 3A) or switched to the 1 1 mixture of buffer and the respective test solution (HP-NaCl, HP-Ri-Ac, HP-6% HES, HP-10% HES and HP-Gel, n = 6 each, protocol 3B).
The dataset comprising 5161 spectrophotometric pH values together with the buffer pH and the concentrations of the chemical species involved was divided into a training set, consisting of those conditions with only one chemical species in addition to the buffer, and a test set of the conditions with multiple chemical species (Fig. 2).
The reaction mixture consisted of crude enzyme solution (0.1 U/ml, 10 μl), in 0.1 M potassium phosphate buffer pH 6.8 (50 μl), and the test sample (20 μl).
The most commonly observed errors were a failure to wait 20 minutes before reading the test result, and adding an incorrect volume of buffer to the test cassette.
Isolated RNA samples were dissolved in 30 μl of REB Buffer from the test kit.
The reaction mixture contained 1.5 mM Fe2+-EDTA solution, 4.4 mM H2O2 and 2 mM luminol in 95% ethanol, 50 mM phosphate buffer (pH 7.4), and the tested samples.
b We incubated 0.4 µM ScLac at 30, 60 and 90 °C for 1 h in the optimal pH buffer and the residual activities were tested against 20 µM DMP.
After pelleting, and detergent lysis of the bacteria to release the scFv-AP, the clarified lysate was diluted in assay buffer and the scFv-AP clones tested directly for binding to their cognate antigens in ELISA by adding substrate.
