Sentence examples for buffer and the suspension from inspiring English sources

Cells were washed with isolation buffer and the suspension was centrifuged at 300 × g at 4°C for 10 min.

Individual flower buds were disintegrated on a microscopic slide by a needle in a drop of citrate buffer and the suspension softened by adding 20 μL of 60% acetic acid.

Briefly, PBLs were thawed with high-salt buffer and the suspension was lysed by three rounds of freeze-thaw cycle and clarified by centrifugation at 15 000 r.p.m. (18 000 × g) for 7 min at 4°C.

When the reaction was finished, bound proteins were eluted by withdrawing the supernatant, adding 500 μL elution buffer and incubating the suspension for 5 min at 800 rpm and 25 °C.

Crude cell lysates of E. coli pHis-DUF15422) were prepared from non-induced and induced (addition of 1 mM Isopropyl-β-D-thiogalactoside [IPTG] and continued growth of 4 h) cultures by harvesting the cells, resuspending the cell pellet in sample buffer, and heating the suspension for 5 min at 95°C.

We then added 12 mL 0.2 x TES buffer and sonicated the suspension five times on ice for 60 s at 70% amplitude (Sonoplus, Bandelin, Berlin, Germany).

DNA was prepared from the 6 isolates of M. tuberculosis by emulsifying 2 3 colonies in 400 μL Tris-EDTA buffer and heating the suspension in a water bath for 40 min at 80°C.

Cell pellets from 10 to 40 dishes (15 cm), were resuspended using buffer B, and the suspension was further homogenized using a dounce homogenizer (15 strokes).

The unbound antibody was washed with FA buffer and the cell suspension subsequently analyzed by FACS.

The column was rinsed with 500  μl of buffer and the cell suspension was added to the column.

The procedure was very easy: the nasal swab was placed in a tube with the buffer, and the resulting suspension was transferred to a lysis tube for DNA extraction.