Sentence examples for buffer and the supernatants from inspiring English sources
The cultures were centrifuged at 18000 × g for 10 min to collect the bacteria, the pellets were dissolved in SDS-PAGE sample buffer, and the supernatants were separated out and then filtered through a 0.22-μm low protein binding filter.
The mitochondrial pellets were lysed in standard 1xRIPA lysis buffer and the supernatants were used as the cytosolic fractions.
This extraction was repeated twice on the remaining pellet using 300 ul of the urea lysis buffer and the supernatants were pooled with the initial extraction to produce the cytoskeleton fraction.
The pellet is extracted once more with 1 mL of Urea Extraction Buffer and the supernatants combined.
These tissues were individually homogenized in PBS buffer, and the supernatants were used for inflammatory cytokine measurement.
Frozen mouse tissues were sonicated in lysis buffer, and the supernatants were collected and used for ELISA.
After extensive washing in GST buffer, the glutathione beads were boiled in sample buffer, and the supernatant analyzed by SDS-polyacrylamide gelectrophoresissis.
The beads were then boiled in SDS sampling buffer, and the supernatant analyzed in SDS-PAGE followed by transfer to PVDF membrane and subsequent immunoblotting with anti-HA mAb and anti-GST mAb.
Samples were washed in binding buffer, and the supernatant was discarded.
The resulting pellet was dissolved in 1.2× non-reducing Laemmli sample buffer and the supernatant was concentrated as described above.
