Sentence examples for buffer and the specimen from inspiring English sources

In order to avoid a reaction between the water fugacity buffer and the specimen, a double-capsule assemblage was designed such that the water fugacity buffer was separated from the specimen by an inner metal jacket permeable to hydrogen and impermeable to other components.

We have scaled the store down and added a plan for a buffer and leaving specimen trees".

Each specimen tube was then rinsed with 2 mL of RBC lysis buffer and the rinse was added to specimens.

The DNA probe (10 µL) was mixed with 70 µL of hybridization buffer, applied to the specimen slide, covered with a cover slip, and incubated in a humidified chamber at 37°C overnight.

After post-fixation for one day in 4%% paraformaldehyde in 0.1 M phosphate buffer (PB), the specimens were cryoprotected using 10%, 200 %and30%0 % glucose in 0.1 M PB.

The primary antibodies were diluted in 0.1% BSA and 0.05% Tween-20 in PBS (diluents buffer) and incubated with the specimens overnight at 4°C.

Secondary antibodies (Molecular Probes) of goat anti-mouse Alexa Fluor 488, goat anti-mouse Alexa Fluor 555 or goat anti-rabbit Alexa Fluor 633 were diluted 1 1000 in blocking buffer and incubated with the specimens for 1 hour at RT.

Modifications consisted of the following: homogenized lysates were incubated in lysis buffer for 1 h at 30 °C prior to loading on the Kingfisher Flex; isopropanol was used instead of ethanol in Wash 1 buffer; specimens were washed twice with Wash I buffer and twice with Wash II buffer; and the temperature of the initial binding step on the Kingfisher was changed to 30 °C for 22.5 min.

After further washes with the above buffer, the specimens were collected and embedded in agarose.

Install the buffer tray and specimen holder in the vibratome.

Following deparaffinization and antigen retrieval in citrate buffer (pH 6.0), the specimens were incubated with primary antibodies followed by horseradish peroxidase (HRP -conjugated secondary antibodies and tHRP -conjugatedwith the 3-amino-9-ethylcarbazole (AEC) + kit (Agilent Technologiesecondaryokyo, Jantibodies