Sentence examples for buffer and the samples from inspiring English sources

Ammonium sulphate was added as a buffer, and the samples were neutralised with NaOH (10 M), and then the mixture was extracted twice with ethyl acetate.

The reactions were stopped by the addition of an equal volume of 2× SDS sample buffer, and the samples were subjected to Western blotting using anti-V5 antibody.

For western blot analysis one to three plates of cardiac myocytes, for each experimental condition, were lysed with RIPA buffer and the samples were separately loaded on SDS-PAGE gel and blotted.

After 3 washes for 5min at 4°C with 1 ml of binding buffer, the beads content was then eluted by Laemmli buffer and the samples were analysed by anti-V5 Western blot.

The beads were then washed three times with RIPA buffer and resuspended in 30 µl of 2×SDS-PAGE loading buffer and the samples were ran on a gel and immunoblotted with the antibodies noted in the results.

Briefly, aliquots containing equal amounts of retinal protein extracts (50 ug total protein) were mixed with 4x SDS gel-loading buffer and the samples were loaded (without reduction or heating) onto 10% SDS polyacrylamide gels containing gelatin (2.6 mg/mL) to detect MMP-9, and fibrinogen (5.5 mg/mL) and plasminogen (50 ug/mL) to detect uPA and tPA.

The surface was rinsed with buffer, and the sample dried.

The HiTrap Protein G HP column (GE Healthcare) was equilibrated with binding buffer and the sample applied.

For high-quality measurements, it is imperative that the temperatures of the buffer and the sample solution be the same.

The fixative was subsequently decanted and replaced with 0.1 M sodium cacodylate buffer and the sample re-suspended and left for 2 hours.

Total proteins were extracted with radioimmunoprecipitation assay lysis buffer, and the sample protein concentrations were determined using a bicinchoninic acid assay.