Sentence examples for buffer and the sample from inspiring English sources

The surface was rinsed with buffer, and the sample dried.

The HiTrap Protein G HP column (GE Healthcare) was equilibrated with binding buffer and the sample applied.

For high-quality measurements, it is imperative that the temperatures of the buffer and the sample solution be the same.

The fixative was subsequently decanted and replaced with 0.1 M sodium cacodylate buffer and the sample re-suspended and left for 2 hours.

Total proteins were extracted with radioimmunoprecipitation assay lysis buffer, and the sample protein concentrations were determined using a bicinchoninic acid assay.

Western blot analysis of purified Flg-4M under non-reducing conditions was performed as above but that β-mercaptoethanol was not included in the sample buffer and the sample was not boiled before loading onto a gel.

Ammonium sulphate was added as a buffer, and the samples were neutralised with NaOH (10 M), and then the mixture was extracted twice with ethyl acetate.

The reactions were stopped by the addition of an equal volume of 2× SDS sample buffer, and the samples were subjected to Western blotting using anti-V5 antibody.

The beads were then washed three times with RIPA buffer and resuspended in 30 µl of 2×SDS-PAGE loading buffer and the samples were ran on a gel and immunoblotted with the antibodies noted in the results.

After 3 washes for 5min at 4°C with 1 ml of binding buffer, the beads content was then eluted by Laemmli buffer and the samples were analysed by anti-V5 Western blot.

For western blot analysis one to three plates of cardiac myocytes, for each experimental condition, were lysed with RIPA buffer and the samples were separately loaded on SDS-PAGE gel and blotted.