Sentence examples for buffer and the reaction from inspiring English sources

After 30 minutes, the reaction was stopped by boiling after addition of 10 µl 2× Laemmli sample buffer, and the reaction products then separated by 15% SDS-PAGE.

For the assay with the purified tubulin (>99% from Cytoskeleton, Inc), the tubulin solution was diluted to a concentration of 10 µg/ml in the assay buffer, and the reaction was performed essentially the same as the cell lysate.

Complex IV (cytochrome c oxidase) activity was determined by the change in absorbance at 550 nm (ε = 19.1 mM−1cm−1) due to the oxidation of reduced cytochrome c. 15 µM reduced cytochrome c and mitochondria were added to the assay buffer and the reaction was followed for 2 minutes.

Subsequently, the coverslips were removed by dipping the slides in washing buffer, and the reaction sequence was continued.

The beads were then resuspended in 125 μL of assay buffer and the reaction mixture was quantified using the Bio-Plex protein array reader.

The chamber was then rinsed with TIRF buffer and the reaction mixture was introduced: 1 μM actin (10% AF488 labeled) and 0.1 1 nM Cy3-diVCA.

Briefly, 0.5 µCi of α[32P]GTP (3000Ci/mmol, PerkinElmer) and 600 nM of purified NS5B were added to polymerase buffer, and the reactions were started by addition of GTP (6.25, 12.5, 25, 50, 100, 200, 300, 500, and 1000 µM), and incubated at 25°C for 15 minutes.

For the in-gel kinase assays shown in Figure 3A,B and Figure 3 figure supplement 2 all steps except the equilibration in reaction buffer and the reactions were carried out together and exactly the same way which allows the autoradiographs to be compared.

The enzyme reaction was started by adding substrate-buffer and the reaction was monitored for 60 min at 37°C as described above.

RCC1 was serially diluted in the GEF reaction buffer, and the GTPase reaction was performed in solution containing 1 μM Ran, 0.5 μM RanGAP, 10 μM GTP, and 1 mM DTT in the reaction buffer with a total reaction volume of 10 μL.

Beads were washed three times with ice-cold buffer B and the reaction was terminated by the addition of 5× SDS loading dye with subsequent boiling at 95°C for 3 min.