Sentence examples for buffer and the proteins from inspiring English sources

The beads were boiled in Laemmli buffer and the proteins resolved by SDS-PAGE (10% gel).

The cells were collected and lysed in RIPA buffer and the proteins were separated via SDS-PAGE.

Following binding, the column was washed 5 times with 10 ml RIPA buffer, and the proteins eluted with high pH (100 mM triethylamine [TEA] pH 11.5).

Beads were washed four times with RIPA buffer, and the proteins bound to the beads were eluted with loading buffer and subjected to SDS-PAGE, as described above.

The beads were washed three times in pull-down buffer and the proteins remaining associated with the beads resuspended in Laemmli buffer.

The membranes were again washed three times with the buffer, and the proteins were visualized using ECLTM western blotting detection reagents (GE Healthcare).

For protein quantification the cells were lysed by a CHAPS-based lysis buffer and the protein was estimated by the DC Protein Assay kit from BIO-RAD according to manufacturer's protocol.

The cell pellet was washed with PBS, lysed cells in lysis buffer and the protein measured using the Bradford assay.

For Western blot analysis, tissues were lysed in RIPA buffer and the protein concentration was determined with Bradford method.

The beads were washed 5 times with lysis buffer, and the protein was eluted by boiling for 5 minutes in 4X sample buffer.

The virus pellet was solubilized in RIPA buffer, and the protein concentration was determined.