Sentence examples for buffer and the products from inspiring English sources

The reaction was stopped by the addition of SDS sample buffer and the products were subjected to SDS-PAGE, followed by a CBB staining.

Reactions were stopped with 2 × SDS-loading buffer and the products were resolved by 15%% SDS PAGE.

The reaction was stopped by the addition of SDS loading dye buffer, and the products were separated using SDS-PAGE gel and autoradiography.

For MGAT1, the column was washed with Con A buffer and the product eluted with 200 mM α-methylmannoside in Con A buffer.

We stopped polymerization by adding SDS buffer and analyzed the products on immunoblots developed with monoclonal antibody Y-18 that recognizes fibrinogen but not fibrin.

After 30 minutes, the reaction was stopped by boiling after addition of 10 µl 2× Laemmli sample buffer, and the reaction products then separated by 15% SDS-PAGE.

After 2 h at 37°C, the reaction was stopped by adding 100 μl 2X loading buffer, and the uncleaved products were separated in 8% poly-acrylamide, 7 M urea, TBE 1X gels.

The sample is mixed with a substrate dissolved in a buffer solution, and the product generated by the hydrolysis of the substrate is monitored during certain time at a linear rate and defined temperature.

The reaction, which was carried out at 60°C for up to 40 min contained 4-NPC in Buffer A, and the product, 4-nitrophenol, was monitored continuously at 400 nm and quantified using a ϵ400 nm of 7500 M-1 cm-1.

Bacterial pellet extracts containing recombinant protein were assayed with GPP (20 μM), E, E- FPP (70 μM) and GGPP (40 μM) in the appropriate buffers [ 32] and the products were subjected to analysis by GC/MS.

e, f, EICs (HR LC ESI-MS) of a mix of 7 (1.7 mM) and buffer (e), or the products of a reaction between 7 (1.7 mM) and Vlm TEDAP (6.5 μM) (f).