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Ligand stocks were prepared in the same buffer and the pH value adjusted appropriately.
Methyl esterified peptides from each sample condition were resuspended in 250 µL of wash buffer, and the pH was adjusted using 10% ammonium hydroxide to ∼3.5.
Peptides were incubated with 100 nm POPC liposomes overnight in pH 8 phosphate buffer and the pH was dropped rapidly to pH 4 by the addition of concentrated HCl.
After the PAH parent compounds had been incubated in SHIME suspension, a 1 mL aliquot of these samples was diluted in 1 mL 0.1 M acetate buffer, and the pH was adjusted to 5 with sodium hydroxide.
The ratio of light emitted with 340 and 380 nm excitation was plotted against the pH values in MES buffer, and the pH calibration curve for the fluorescence probe was generated from the plot using Microsoft Excel.
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The molecular crowding agents or cosolvents in 10, 20, 30, and 40 wt % (w/v) were added to the buffers and the pH adjusted as necessary for a range of 5.3 to 8.0.
The size and morphology of these nanostructures could simply be controlled by varying the ratios of two components, the type of the buffer solution, and the pH of the solution.
NaOH and HCl were used to adjust the pH of buffer solution and the pH values were determined by a bench top pH-meter (METTLER TOLEDO, China).
Selected ligands were studied to correlate the elution yield to the salinity of the binding buffer and the elution pH.
Before use, the buffer was reoxygenated and the pH was readjusted.
Stock solutions of 7 were made up in 100 mM Na2HPO4 buffer (pH ∼9), and the pH was slowly adjusted to 7.3 via the addition of 2 μL aliquots of a 1 M NaOH solution.
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