Sentence examples for buffer and the mixture from inspiring English sources
In step 2, annealed oligomers were mixed with various purified proteins in 1× T4 ligation buffer and the mixture was incubated in a water bath for 20 min at 30 °C.
The supernatant fraction was mixed with 1 ml of Ni-resin equilibrated in binding/wash buffer and the mixture was incubated for 1 h at 4 °C.
After this, 1 mmol of maghemite nanoparticles (γ-Fe2O3) were mixed with the BSA containing buffer and the mixture was vortexed for 1 h at room temperature.
All samples were incubated with and without excess InO diluted in serum containing buffer, and the mixture was added to a microtiter plate.
Whole-cell lysate proteins were mixed with an equal volume of electrophoresis sample buffer, and the mixture was then boiled for 10 min.
Briefly, 200 µl of an EDTA-treated plasma sample was added to 2 ml of lysis buffer and the mixture was incubated for 10 min at room temperature.
STIV (10 TCID50/ml) was incubated with each aptamer (200 nM) in binding buffer and the mixtures were passed through a pre-wetted 0.1-μm PVDF filter (EMD Millipore).
The assays employed 10 μL of diluted centrifugation supernatant and 90 μL of the respective pNP (0.5 mM, diluted in citrate buffer), and the mixtures were incubated for 10 min at 50°C.
2-DE was performed by a previously described protocol [ 56]. 500 μg of the second part of the protein sample of GV and ESV were dissolved in the lysis buffer, and then the mixture (1 μl mixture/4 μl rehydration buffer) was added into a rehydration buffer (8 M uera, 2% CHAPS, 0.001% bromophenol blue, 45 mM DTT, and 0.5% Biolyte, pH 3 10) for the 2-DE runs.
DNA from groups of 50 larvae was extracted using a rapid DNA preparation protocol, which consisted of grinding the frozen tick larvae in PCR buffer and boiling the mixture for 5 min.
DNA was cleaned with a ZR-96 DNA Clean and Concentrator-5 kit (Zymo Research) by mixing the previous reaction with 560 µl Binding buffer and dispensing the mixture into a Zymo-Spin column.
