Sentence examples for buffer and the membrane from inspiring English sources

The RNA was then transferred to a nylon membrane (Ambion) in the same buffer and the membrane was baked for 30 min at 80°C.

Pellets were resuspended in an isotonic buffer and the membrane vesicles were isolated with spinning steps of 4000  g and 100.000  g.

After prehybridization, the purified labeled probe was added to the prehybridization buffer and the membrane was hybridized at 42°C for 12 – 18 h.

Unbound antibodies were removed by 2 min washing in detection buffer and the membrane was evenly covered with 1 ml of CSPD diluted 1/100 v/v in detection buffer.

Un-incorporated label was washed away with five 10to6060 minute washes with 0.03 M sodium citrate, pH 7.0, in 0.3 M sodium chloride (SSC) buffer, and the membrane was then fixed in 100% ethanol.

Three washes for 5 min each time were performed in TTBS buffer and the membrane was incubated with horseradish peroxidase-conjugated anti-mouse (Santa Cruz Biotehnology) for 2 h at room temperature, diluted 1 2000 in 5% blocking reagent (Amersham) in TTBS buffer.

This result would suggest that the buffer condition (pH 6 MES buffer) and the membranes being in a vesicular form (no detergent) permit endoproteolysis, and protease inhibitors prevent degradation of the subsequent tripartite complex.

The signal was developed after washing with TBST buffer and exposing the membrane to X-ray film.

For amino-terminal sequencing of the midgut proteins, the proteins were electro-blotted to PVDF blot paper in a Xcell II Mini-Cell with transfer buffer (Invitrogen) and the membrane stained with 0.025% Coomassie Blue (R) in the absence of acetic acid.

The pellet was lysed in 1X lysis buffer and the pure membrane fraction collected after centrifugation at 14,000 rpm for 10 min at 4°C.

2. Discard the washing buffer and rinse the membranes with 20 mL of blocking solution [Odyssey Blocking Buffer (LI-COR Bioscience, Lincoln NE, USA) and PBS 1× - (1 1)] for 15 minutes on a rocking platform.