Sentence examples for buffer and the level from inspiring English sources

Cells were then harvested in lysis buffer, and the level of phospho-AKT was quantified according to the supplier's protocol.

The control Renilla luciferase signal was recorded after the subsequent addition of 50  μl of Stop&Glow buffer, and the level of expression given as a ratio.

For analysis with flow cytometry experiments, the MDA-MB-231 (with or without eGFP) cells grown in 12-well plates (1.0 × 10 cells per well) were lifted with cell dissociation buffer, and the level of nanoparticle uptake or expression of eGFP was determined by fluorescence-activated cell sorting (FACS) analysis on a FACScalibur flow cytometer (BD Bioscience).

For the next stage, the input signal is the output concentration of the previous Buffer and the threshold level is the half maximal output level in the previous one.

Then, the cells were lysed with Assay/Lysis buffer (Thermo Fisher Scientific) and the level of cyclic AMP in the cell lysate was determined by using the cAMP-screen® assay (Thermo Fisher Scientific) according to the manufacturer's instructions.

Briefly, cells were resuspended in NAD/NADH extraction buffer and the OD595-levels were determined before disrupting the cells using a bead mill.

The extent to which each mechanism dominates is dependent on the actual type of stationary phase used and the buffer conditions, including the level and type of organic content, the type and concentration of salts and the pH [ 10].

The extent to which each mechanism dominates is dependent on the actual type of stationary phase used and the buffer conditions, including the level and type of organic solvent, the type and concentration of salt, and the pH [ 47].

The pattern adaptive last-level cache consisted of three major parts: the last-level cache, the first-level prefetching buffer (FLPB) and the second-level prefetching buffer (SLPB).

In such a scenario, the utility of a node is defined on the basis of available buffer space and the battery level.

Then, cells were centrifuged and washed four times with FACS buffer before incubation with the corresponding FITC-conjugated secondary antibody for 30 min. Then, the cells were centrifuged and washed four times in FACS buffer, and the cell surface CD44 expression level was measured by flow cytometry (BD Accuri C6 flow cytometer).