Sentence examples for buffer and the gel from inspiring English sources

The lower chamber was then replenished with fresh buffer and the gel was run for an additional 45 minutes.

It was then replaced with fresh developing buffer and the gel was incubated at 37°C for 16 h.

Excess mAb was removed by washing five times with 1.0 mL of coupling buffer, and the gel was allowed to stand in 4.0 mL of 0.1 M Tris-HCl buffer (pH 8.0) for 2 h at room temperature.

Excess ligand was eluted with 20 ml of coupling buffer and the gel was incubated in 0.1 M Tris-HCl buffer pH 8.0 for 2 hours at room temperature.

The fluid interface migrates at the same velocity (<1% difference), regardless of whether the well is filled with buffer or gel precursor, indicating that the position is dictated by hydraulic pressure alone and differences in viscosity and surface tension are negligible between the buffer and the gel precursor.

After amplification, 8  μl of the reaction mixture was removed and analysed by means of electrophoresis through 2.0% agarose gels in Tris-borate-EDTA buffer, and the gels were then stained with ethidium bromide.

However, out of seven investigated parameters, just four showed a significant effect on some proteins, namely the parameters of: destaining time, staining temperature, changes of detergent additives (SDS and LDS) in the sample buffer, and the age of the gels.

The stacking gel is 5% acrylamide in 62.5 mM Tris-HCl pH 6.8 buffer and the running gel is 10% acrylamide in 375 mM Tris-HCl pH 8.8.

The new Tris buffer was replaced and the gel was incubated for 4 hrs at 37°C to allow caseinolysis occur.

Add 1ml 2X Lysis Buffer to the gel and pipette up and down.

To make a cell lysate, add 1ml 2X Lysis Buffer to the gel and pipette up and down.