Sentence examples for buffer and the final from inspiring English sources

Two included 10% glycerol in the buffer and the final measurement was done in the absence of glycerol to flatten the baseline for the refractive index measurements.

At the completion of binding, the surface was washed with running buffer and the final baseline was recorded.

Drugs were diluted in Tris-citrate buffer, and the final volume in the well was 150  μL.

The tissues were dissolved in RIPA lysis buffer, and the final protein concentration (2  μg/ μL) was quantified using the BCA kit.

The Protein-G-Sepharose-anti-myc antibody complex was washed five times with RIPA buffer, and the final pellet was mixed with 2× SDS/PAGE sample buffer (Bio-Rad) containing 5% (v/v) 2-mercaptoethanol.

In this case, the reaction was stopped by diluting the sample 500 times in buffer, and the final sample was checked by single-molecule analysis (see Figure S3 A).

All wash buffers and the final resuspension buffer included × 1 protease inhibitor cocktail (Roche, Switzerland), NaF (5 m M), and Na3VO4 (200 μ M).

All of the wash buffers and the final resuspension buffer included 1 × protease inhibitor cocktail (Roche, Basel, Switzerland), NaF (5 mM), and Na3VO4 (200 μM).

Imidazole was removed from the purified proteins by buffer exchange into Buffer A, and the final protein was flash frozen with liquid nitrogen and stored at −80 °C.

The protein was purified from this lysate using Softlink Avidin Resin and HiTrap heparin columns as described for bioUvrD, except that buffer A was used in place of buffer C and the final protein solution was dialysed against storage buffer.

The oocytes were then rapidly washed five times with ice-cold uptake buffer and, after the final wash, each egg was transferred into a scintillation vial and homogenized in 100 μl of 2% (w/v) SDS by vigorous vortex-mixing.