Sentence examples for buffer and the embryos from inspiring English sources

Worms were dissected in M9 buffer and the embryos were mounted on 2% agarose pads for image acquisition.

They were then treated with 10 µg/mL proteinase K (Roche diagnostics) at 37° for 30 min, washed with PBS, and acetylated using 0.1 M triethanolamine-HCl (pH 8.0) and 0.25% acetic anhydride for 10 min. This solution was gradually replaced by hybridization buffer, and the embryos were hybridized with a labeled probe (100 ng/ml concentration) at 60° overnight.

To take the eyes from E15 and E18 embryos, pregnant dams were anesthetized with chloral hydrate, perfused through the hearth with 4% paraformaldehyde in 0.1 phosphate buffer (pH 7.4) and the embryos were removed after surgical hysterotomy.

After thorough washing with buffer B, the embryos were incubated in Hoechst 33258 stain before mounting for confocal microscopy.

Cocoons were collected 48 h after deposition and transferred to Petri dishes in CMF buffer [Calcium Magnesium Free solution], and the embryos were carefully dissected from their cocoons in a mitosis-rich developmental stage (192 to 288 h after fecundation).

After incubation in the hybridization buffer, the embryos were washed twice in Solution I (50% formamide, 2× SSC, 1% SDS) at 70°C, twice in Solution II (50% formamide, 1× SSC) at 70°C and 3 times in PBST at RT.

On day 4 postinfection the eggs were opened, and the embryos were fixed in 4% buffered formaldehyde solution.

Briefly, buffer D2 was added and the embryo was crushed and ground thoroughly with a pipette tip.

Antibodies and antiserum were diluted in blocking buffer and embryos incubated overnight in this solution at 4 °C.

Primary antibody was washed off by ABS buffer, and embryos were incubated overnight with secondary antibody goat anti-mouse conjugated with Alexa-488 (1∶250, Molecular Probe)[Tucker et al, 2006].

Adult worms were dissected in M9 buffer, and embryos were mounted onto 2% agarose pads for imaging.