Sentence examples for buffer and the concentration from inspiring English sources

A face-centred central composite design was applied to study the effect of the pH, the molarity of the running buffer, and the concentration of the micelle-forming agent on the separation properties.

The isolated DNA was eluted in 100 μL of elution buffer, and the concentration was determined by a Nano Drop spectrophotometer (Nano Drop Technologies, Wilmington, DE, USA).

After extraction, the DNA was resuspended in 50 μL of 1× Tris EDTA (TE) buffer, and the concentration was estimated by comparison to a known standard on a 0.8% agarose gel.

For TGN46 staining, goat serum was omitted from the blocking buffer and the concentration of human IgG raised to 40 µg/ml.

The genomic DNA samples were kept in TE buffer, and the concentration was adjusted to 10 to 20 ng/μl.

Cells were lysed in lysis buffer and the concentration of protein was determined by the Bradford dye method (Bio-Rad Laboratories).

Solutions with the indicated concentration of SbpA in self-assembly buffer and the indicated concentration of CaCl2 were incubated at 25 °C for at least 16 h.

However, the standard SBF formulation, first, contains the tris/HCl buffer, and, second, the concentration of hydrogencarbonate (4.2 mM) is only a fraction of that in blood plasma (27 mM) (Kokubo et al. 1990).

For protein extraction, T lymphocytes from the recipient mice were treated with RIPA lysis buffer (Beyotime), and the concentration of protein was assessed by using a BCA protein assay kit (Beyotime).

The DNA obtained was eluted in 50  μl of buffer AE, and the concentration and purity of the extracted DNA were assessed by spectrophotometry.

The crude purified solution was run through a HiPrep Q FF 16/10 strong anion-exchange chromatography column (GE Healthcare) with TD buffer, and the NaCl concentration was linearly increased to 1 M.