Sentence examples for buffer and the cells from inspiring English sources

Small aliquots were washed with the buffer and the cells quantified on FCM as the way to determine the effector to target cells' ratios (ETR).

Then, the samples were washed with 2 mL permeabilization buffer, and the cells were suspended in Macs buffer for flow cytometric analysis [15].

With incubation in dark at room temperature for 15 min, each sample was added 400 μL of binding buffer, and the cells were immediately analyzed by BD FACSCalibur (BD Biosciences, Mountain View, CA).

(a) Negative control cells treated with PBS buffer and the cells incubated with PEGylated Au DENPs (at the Au concentration of 100 μM (b) and 300 μM (c) for 24 h).

Primary antibodies were diluted in blocking buffer and the cells were incubated overnight.

For isolation of total cellular proteins, the bacterial pellets were resuspended in PBS buffer and the cells were broken by sonification.

The pellets were suspended in Tris buffer and the cell walls were disrupted in a Microfluidizer M-110L.

The unbound antibody was washed with FA buffer and the cell suspension subsequently analyzed by FACS.

Cells were then lysed in Laemmli buffer and the cell lysates analyzed for apoptin phosphorylation by western blotting.

Formaldehyde-treated C18-4 cells were re-suspended in SDS lysis buffer, and the cell lysates were sheared by sonication.

The column was rinsed with 500  μl of buffer and the cell suspension was added to the column.