Sentence examples for buffer and the amount from inspiring English sources

After 48 h, samples were lysed using 1x Passive lysis buffer, and the amount of firefly luciferase and Renilla luciferase was quantified using the Dual-Luciferase Reporter Assay System Promega Corporationn, Masison, WI), as we described recently [15].

After immunoprecipitation, beads were washed three times with lysis buffer and the amount of HCF101 Myc-associated radioactivity was measured by scintillation counting.

Protein bound to the beads was washed three times in lysis buffer and the amount of bound fusion protein was estimated using Coomassie-stained SDS gels.

Tissue samples or myofibroblasts were homogenized in ice-cold lysis buffer, and the amount of protein was determined by a protein assay (Bio-Rad Laboratories).

Colospheres, spheroids and xenograft tissue were lysed with zymogram sample buffer and the amount of protein was estimated using the Bradford method (Biorad Dc Protein Assay; Bio-Rad Laboratories).

The ratio of oral secretion to Mes-NaOH buffer and the amount of OS solution added to mechanical injured (MI) tomato plants were as reported in previous studies [ 3, 6, 10, 11, 32].

aRNA was fragmented using 5x fragmentation buffer and the total amount of fragmented biotinylated aRNA used per chip was 6.5 μg.

Two days after cell seeding at the density of 6.0 × 10 cells/100 mm cell culture dish, the cells were lysed with lysis buffer and the amounts of EGFR and HER2 in the lysate were quantitated using Human Total-EGFR and Total-ErbB2, DuoSet IC ELISA kits (R&D Systems).

Finally, Au-citr CTAB -TbADH bioconjugAu-citr CTAB -TbADHAu-citr CTAB -TbADHhosphate bioconjugate.0, and the amount of immobilized enzyme was calculated from the difference in the protein amount before and after immobilization process.

The second method, adopted by us from relatively small prior studies, employs citrate-containing replacement solution in predilution mode of CVVH, which is isotonic and acts as an anticoagulant and buffer, so that the amount of bicarbonate equivalents and control of acid base status are similar to that employed when lactate- or bicarbonate-buffered solutions are used [ 3, 8, 10- 124 24- 26].

Banks hold capital to provide a buffer between the amount they owe creditors like depositors and bond holders and the value of their assets.