Sentence examples for buffer and the absorbance from inspiring English sources
The washed pellet was resuspended in PUM buffer and the absorbance was adjusted to ∼0.7 OD at 600 nm.
Peptides were added to a 500 µl quartz cuvette containing 10 µg of LUVs in a HEPES 10 mM pH 7.4 buffer and the absorbance was followed during 20 min after peptide exposure.
Peptides were added to a 500 µl quartz cuvette containing 10 µg of LUV in a HEPES 10 mM pH 7.4 buffer and the absorbance was followed during 20 min after peptide addition.
Samples were diluted with dilution buffer and the absorbance taken at 405 nm.
The fecal extracts were diluted 1 50 with incubation buffer and the absorbance was read at 450 nm in a microtiter plate reader.
The proteins were exchanged with these buffers and the absorbance spectra collected at approximately 2 μM with a PerkinElmer Lambda 25 UV vis spectrometer.
Briefly, 10 μL of diluted plasma with phosphate buffer (10 mM, pH = 7.4) was mixed on 96-well plate with 190 μL of BCS 0.25 mM (dissolved in phosphate buffer), and the initial absorbance (Ai) was measured at 490 nm.
The dye was extracted with 10% cetylpyridinium chloride monohydrate (Sigma-Aldrich) in 0.1 M phosphate buffer (pH 7.0) and the absorbance was read at 550 nm (Perkin Elmer Victor X3 microplate reader).
The extract was then combined similarly with 0.025 M sodium acetate buffer pH 4.5 and the absorbance was measured at the same wavelengths after being allowed to stand for 15 min.
After centrifugation, 0.5 mL of the supernatant was added to 4 mL of ice-cold 0.1 mM 5,5-dithiobis[2-nitrobenzoic acid] solution in 1 M phosphate buffer (pH-8) and the absorbance was measured at 412 nm.
Biliverdin-containing kit assay buffer (50μL), biliverdin reductase solution (200μL), and NADPH solution (0.24mg mL-1 NADPH in assay buffer, 750μL) were combined and the absorbance spectrum between 300-800nm was measured at 0, 15, 30, 45, 60, 90, 145, 240 and 360min using a SpectraMax Plus384 Absorbance Microplate Reader (Molecular Devices, Sunnyvale, CA, USA).
