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Each serum sample was serially diluted with HBS-EP buffer and tested on both chips at a standard association/dissociation time of 300/300 s.
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An aliquot (10 μl) diluted in equal volume of double strength SDS loading buffer was tested on SDS-PAGE to confirm induction using prestained protein marker as a guide of protein size.
This assumption can be tested by isolating single buffer components and testing a concentration range against the normal buffer conditions.
The efficiency of this buffer to prevent DNA degradation was tested on 10 samples at room temperature for up to 24 months.
Therefore, composites were tested on setting time and mechanical strength as well as subjected to phosphate buffered saline (PBS) and enzyme containing medium.
The rinse buffer for (ii), the ligand/additive screen, was the same composition as (i) with the replacement of Tris by the optimal buffer determined from the buffer screen (Hepes pH 6.8, see Results section) at 4 °C, The rinsed beads were immediately aliquoted (16 µl aliquots) and tested by addition of 2 µl of ProteoPlex ×88 buffers (or ×88 ligands) screens at 4 °C.
SMP buffer outperformed other buffers tested, such as 10% PEG 8000, 15% glycerol, protoplast buffer with lysozyme omitted, and SMP buffer supplemented with 15% glycerol (data not shown).
Two different concentrations of ammonium formate buffer were tested (5 mM and 10 mM) for their effect on separation, resolution and peak symmetry.
The effect of the dilution in the Panbio buffer was tested prior to the use on patient samples and did not show any influence on the results (results not shown).
The buffer rod tested has tapered shape and cladding on the side wall as a further suppression of trailing echo.
The supplemented buffer corresponded to 90% Taq storage buffer and we also tested a further dilution to 50% storage buffer.
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CEO of Professional Science Editing for Scientists @ prosciediting.com