Sentence examples for buffer and subsequently incubated from inspiring English sources

Blots were incubated with indicated primary antibodies, washed three times in 1× TBS-T buffer, and subsequently incubated with HRP-conjugated secondary antibodies (ZSGB-BIO, Cat. No. ZF0136, Cat. No. ZF0312).

The samples were then washed with TBS buffer and subsequently incubated with secondary antibody for 30 min.

Slides were washed twice with PBS buffer, and subsequently incubated with goat serum for 30 min at room temperature.

For digestion, pulverized tissue was directly resuspended in a hypotonic digestion buffer and subsequently incubated with Benzonase or Cyanase at different concentrations.

The cells were washed twice with binding buffer and subsequently incubated at 37°C for 10 min in 1.0 ml binding buffer.

Following incubation with the respective primary antibody, sections were thoroughly rinsed in wash buffer and subsequently incubated in peroxidase coupled secondary antibody (30 min) (DAKO K4001, DAKO K4002).

For this purpose ion exchange-purified Dds were dialyzed overnight at 4°C against various buffers and subsequently incubated at 30 or 37°C.

Arrays were blocked in 100  μL Protein Array Blocking Buffer (Whatman) and subsequently incubated with 90  μL of 1 : 10 diluted serum, eluted DBS, or pooled PAPP-A and f β-hCG standards.

On the day of experiment, the cells were washed with 1 × phosphate buffered saline and subsequently incubated with 25  μM DCFH-DA in the dark for 45 min.

After electrophoresis, gels were washed twice with distilled water and once with 50 mM Tris buffer (pH 6.8) and subsequently incubated overnight in the same buffer at 37 °C.

For E-cadherin detection, sections were subjected to antigen retrieval for 40 min at room temperature in 0.01  M sodium citrate buffer, pH 6.0 and subsequently incubated for 60 min with a monoclonal E-cadherin antibody (VENTANA no.790-4497, Vienna, Austria).