Sentence examples for buffer and subsequently extracted from inspiring English sources

The pellet was washed once in the hypotonic lysis buffer and subsequently extracted with high-salt buffer (10 mM Tris, pH 7.5, 500 mM NaCl, 5 mM EDTA), washed again and then finally extracted in carbonate buffer (100 mM sodium carbonate, pH 11.0).

Desorption of Cry1Ab proteins from paddy soils was performed by washing with sterile Milli-Q water (H2OMQ), and subsequently extracted with an extraction buffer.

Perl scripts were written to remove low-quality sequences, extract valid ditags and subsequently extract individual tags.

Pellets were incubated in a buffer containing proteinase K, and mtDNA was subsequently extracted using phenol chloroform followed by ethanol precipitation and resuspension in dH2O.

Symbiont cells for molecular analyses were isolated from the salt-water "blastate" via centrifugation in 50 mL tubes at 2000 3000 g for 5 min, preserved in DMSO Buffer (20% dimethyl sulfoxide and 0.25 M ethylenediaminetetraacetic acid [EDTA] in NaCl-saturated water [36]) and nucleic acids subsequently extracted following Thornhill et al. [31].

The corresponding plasmid was subsequently extracted and analyzed by sequencing.

The software subsequently extracted sentences, phrases, and individual terms to generate a text corpus.

Adult samples were subsequently extracted in 50 μl lysis buffer with 200 μl/ml Proteinase K (final concentration).

Proteins were extracted by homogenisation in 0.8 ml of pH 7.5 Tris-EDTA buffer, and subsequently quantified and normalised using the Lowry method [27].

For the production of RPPA chips, the tumor lysates were adjusted to 3 mg/ml protein concentration in CLB1 buffer and subsequently diluted tenfold with printing buffer CSBL1.

Afterwards, beads were collected and washed three times with lysis buffer and subsequently eluted with sample buffer and boiled.