Sentence examples for buffer and subsequently analyzed from inspiring English sources
Bound proteins were eluted by boiling in SDS-PAGE sample buffer and subsequently analyzed by Western blotting using the indicated antibodies.
Reaction was stopped by adding 10 µl of SDS-PAGE loading buffer and subsequently analyzed with gel-electrophoresis and immunoblotting using monoclonal anti O-GlcNAc anti O-GlcNAc
A sample aliquot was removed, APC added (2.0 nM), aliquots taken at selected time points and quenched into denaturing sample preparation buffer, and subsequently analyzed by Western blotting.
Equal amounts of the cells (by OD600) were harvested and lysed by boiling in 1 × SDS-PAGE loading buffer, and subsequently analyzed with SDS-PAGE.
A sample aliquot was removed, APC added (2.0 or 20.0 nM), aliquots taken at selected time points and quenched into denaturing sample preparation buffer, and subsequently analyzed by Western blotting.
Washed cell pellets from bone marrow were resuspended in 200 μl antibody cocktail and incubated at RT for 10 min, washed in buffer, and subsequently analyzed by flow cytometry.
The samples were then precipitated by trichloroacetic acid (TCA), resuspended in boiling sample buffer, resolved by SDS-PAGE and subsequently analyzed by immunoblotting with appropriate antibodies.
The sample buffer was carefully removed and run on a 10% SDS-PAGE gel and subsequently analyzed utilizing the phospho-specific antibodies pStat3 (Tyr 705), p-c-Src (Tyr 416), pErk 1/2 (Thr202/Tyr204) and pAkt (Ser 473).
Other top-down SCRaMbLEd plasmids were initially analyzed using restriction digestion and subsequently analyzed using a primer walking sequencing method.
