Sentence examples for buffer and subjected into from inspiring English sources

Lyophilized conditioned media were normalized to the number of cells mixed with non-reducing LaemmLi buffer and subjected into 10% SDS-polyacrylamide gels containing 1 mg/mL gelatine in the presence or absence of BP.

E2F-3T3 cells (2×105) were boiled in reducing Laemmli buffer and subjected to SDS-PAGE.

Samples for western blotting were collected into 1xSDS-sample buffer and subjected to immunoblotting with anti-c-Myc (Santa Cruz Biotechnology; sc-40 or Millipore; 06-340), anti-FLAG (Sigma; 8592); anti-aPKC (BD Biosciences; 610207), or in-house prepared pSer218-FoxO1 anormalizedzed to anti-α-tubulin (Cell Signaling Technology; 2125).

After treatment, cells were scraped into a Laemmli buffer and subjected to SDS-PAGE using 4%20%% pre-cast gels (Life Technologies), and then semi-dry transferred to PVDF membranes (Millipore, Billerica, MA).

To determine the expression of p53, and Hsp90s in intact nuclear fractions, the nuclear pellet was lysed into 1 × SDS sample buffer and subjected to denaturing gel electrophoresis followed by immunoblotting as described above.

After extensive washing with NETN buffer, the beads were eluted with SDS sample buffer and subjected to western blot analysis.

The beads were eluted with SDS sample buffer and subjected to SDS-PAGE.

The eluted protein was resuspended in Laemmli buffer and subjected to SDS-PAGE.

Complexes were washed, eluted from the beads with SDS buffer, and subjected to RNase and proteinase K treatment.

After sonication, the samples were heated at 100 °C for 10 min in SDS sample buffer and subjected to western blotting analysis.

The beads were then washed 5 times with IP buffer, incubated at 95°C for 10 min with 2× Laemmli buffer and subjected to SDS-PAGE.