Sentence examples for buffer and stored on from inspiring English sources

The mitochondrial pellet was then suspended in 250 μl STE + BSA buffer and stored on ice.

The washed cells were resuspended in electroporation buffer and stored on ice.

Finally, cells were washed once in PBS and re-suspended in FACS flow buffer and stored on ice until analysis by flow cytometry on a FACS Canto II.

The final pellet was resuspended in approx. 1 1.2 ml of STE buffer and stored on ice for up to 6 h.

The supernatant was centrifuged at 16000  g for 75 min at 4°C, and the pellet, containing the membrane fraction, was washed with 2 ml of homogenization buffer and stored on ice.

Lysates were centrifuged at 1000  g for 2 min at 4°C, and the pellet, containing the nuclear fraction, was washed with 2 ml of homogenization buffer and stored on ice.

Before each experiment, aliquots of the LC were thawed to room temperature and immediately passed through a PD-10 gel-filtration column equilibrated with Buffer P. The protein was collected in Buffer P and stored on ice.

After another centrifugation for 10 min at 10,000 g, the pellet was resuspended in isolation buffer (without EGTA) and stored on ice until analysis.

Bacteria were removed from the incubator at log growth phase and, prior to injection, L. monocytogenes cultures were diluted to the desired optical density (OD) 600 in phosphate buffered saline (PBS) and stored on ice.

The cells were finally resuspended in transport buffer (10 OD600 ml-1) and stored on ice.

Reactions were stopped by the addition of 1x Laemmli buffer plus 100 mM DTT, and stored on ice until SDS-PAGE analysis.