Sentence examples for buffer and stored in from inspiring English sources

To complete the immobilization, the beads were washed with 6 mL washing buffer and stored in 500 μL immobilization storage buffer.

The membranes were washed three times with the pre-cold lysis buffer and stored in PBS solution (pH 7.4) at −20 °C until analysis.

The purified mitochondria were re-suspended in isolation buffer and stored in liquid nitrogen until lipid analysis was performed.

The reaction was stopped with several washes in Tris buffer, and stored in this buffer before processing for EM.

FLAG-tagged protein complexes were eluted with 80 µl 3XFLAG Peptide (Sigma) solution (0.225 mg/ml in lysis buffer) and stored in the −80°C freezer.

The beads were subsequently washed five times with wash buffer and stored in PBS containing 0.1% BSA, 0.01% Triton X-100 and 0.02% NaN3 at 4°C.

The cells were then washed twice in Perm/wash buffer I and stored in that same buffer on ice until the time of acquisition The phospho-antibodies were from BD Biosciences, anti-phospho-Stat3Y705 (clone 4/P-Stat3), anti-phospho ERK1/2 T202/T204 (clone 20a), anti phosphor Akt S473) (clone F29-763).

A 15  μmol ·L−1 HSA stock solution was prepared in Tris-HCl buffer solution and stored in the dark at 4°C.

Samples were then washed in phosphate buffered saline and stored in 70% ethanol until scanning.

Mandibles were dissected from the skull, fixed in 4% paraformaldehyde for 24 h, rinsed with phosphate buffered saline, and stored in 70% ethanol-diethyl pyrocarbonate.

Buffer was removed, beads were washed excessively with 50 mM sodium phosphate buffer, pH 7.5, and stored in this buffer at 4°C until further use.